DATE OF BIRTH:   14.09.1977  

PLACE OF BIRTH: Tbilisi, Georgia

PHONE, E-MAIL: (+995) 577 947465; n.kulikova@agruni.edu.ge; nkulikova@gmail.com

DEGREES:    

2000-2003, January - PhD in Immunology, Javakhishvili Tbilisi State University

1998-2000, June - MSc in Immunology, Javakhishvili Tbilisi State University

1994-1998, June -  BSc in Biology, Javakhishvili Tbilisi State University

APPOINTMENTS:

2013, May – till now – Full Professor, Agricultural Universty of Georgia (www.agruni.edu.ge)

2013, April – till now – Head of the Cellular Immunology Laboratory, Agricultural Universty of Georgia

2013, February – till now – Cytometry Consultant, “Pharmax” Ltd.

2007, September – 2013, April - Head of the Immunology/Microbiology Laboratory, Javakhishvili Tbilisi State University

2007, January – 2007, July - Invited Lecturer, Division of Immunology and Microbiology, Javakhishvili Tbilisi State University

2002, October – 2007, September - Senior Expert, Immunodiagnostic Centre “Immunodiagnostics XXI”

2006, January – 2007, September – Research Applications Expert, Coramedi Ltd. (BD Biosciences representative company in Georgia)

2004, October - 2006, December – Researcher, Laboratory of Immunoregulation/Immunobiotechnology, Javakhishvili Tbilisi State University

2005, September – 2005, December - Invited Lecturer, Department of Immunology, Javakhishvili Tbilisi State University

2001, June – 2004, September – Senior Research Technician, Laboratory of Immunoregulation/Immunobiotechnology, Javakhishvili Tbilisi State University

             

TEACHING EXPERIENCE:

2011 –2013 - Course Leader for MSc in Medical Molecular Biology By Flexible Learning – Collaborative Degree – University of Westminster, London, UK and TSU, Tbilisi, Georgia

2008, March-2008, July – BSc module “Molecular Diagnostics” and MSc module “Molecular Diagnostics”

2007, September-2007, February - MSc module “Advanced Immunology”

2007, January – 2007, July - BSc module “Molecular Diagnostics”

2005, September – 2005, December – MSc module “Molecular Immunology” and MSc module “Advanced Immunology”

I’ve supervised 7 MSc projects.

PARTICIPATION IN GRANT PROJECTS:

2013, September – 2013, December, Individual Mobility Project “Study of defense mechanisms against Aspergillus fumigatus (AF), using airway 3-dimensional (3D) cell culture  model” , Shota Rustaveli National Science Foundation, Recipient

2010-2013 European Commission, Directorate-General for Education and Culture,

Tempus, project 158627-TEMPUS-UK-TEMPUS-JPCR “Development of e-learning and distance learning courses in Biomedical Sciences in Southern Caucasus”, Regional Coordinator

 2008 - 2010  – Georgian National Science Foundation  (GNSF) project “Anti-viral responses and regulatory function of CD4+ cytotoxic T cells in  patients with B cell chronic lymphocytic leukaemia (B-CLL)”, Project Manager

2008 - 2010 – Georgian National Science Foundation (GNSF) project “Development of anti-tumour and immunocontraceptive epitope-specific vaccine based on the mutant molecule of the β-chain of human chorionic gonadotropin (hCG)”, Senior Researcher

2007, December – 2009, December - European Commission, Directorate-General for Education and Culture,Tempus-Erasmus Mundus,  project CD_JEP-27218-2006 (GE): “Developing New Applied Biosciences and Biotechnology Curricula at Tbilisi State University”, working group member

2006-2007 -  European Commission, Directorate-General for Education and Culture,

Tempus-Erasmus Mundus, Individual Mobility Grant- IMG-GEO1004-2006: “Training in implementing computer-assisted assessment and e-learning tools in higher education”, Recipient

2002-2006 - EU INTAS project “Immunotherapy of B cell chronic lymphocytic leukaemia (B-CLL)”, Senior Researcher

2003-2005 - European Commission, Directorate-General for Education and Culture,

Tempus-Erasmus Mundus, Multiplier project: "Establishment of the centres of excellence in Biomedical and Veterinary education in Southern Caucasus", web-master

2001-2003 – ISTC project “In Vitro Study of Mechanisms of Intracellular Responses to Low-Dose and Low-Dose Rate Exposure to Cr (VI) Compounds”, Researcher

2001-2003 – European Commission, Directorate-General for Education and Culture,

Tempus-Erasmus Mundus, Networking project: "Creation of the Network of the regional universities for the development of biomedical education in Georgia", trainer/teacher

1999-2001 - EU INTAS project “New approaches to the immunotherapy of B-Chronic Lymphocytic Leukaemia: use of bispecific antibodies”, junior researcher

1996-2000 - European Commission, Directorate-General for Education and Culture,

Tempus-Erasmus Mundus, “Development and Restructuring of Higher Education in Immunology in Georgia”, student/trainer

TRAINING:

Technology Enhanced Learning 2010, October	London, UK University of Westminster
IT Technologies and Academic Regulations 2010, November	London, UK University of Westminster
Curriculum Development 2010, July	Alicante, Spain University of Alicante
Virtual Learning Environment (VLE): Usage and Administration 2007, February -March	London, UK University of Westminster
Flow Cytometric Methods for the Dignostics of Leukaemia/Lymphoma: International Guidelines 2006, July	Heidelberg, Germany BD Biosciences Eastern Europe Central Office
CBA technology for research applications 2006, May	Istanbul, Turkey BD Biosciences Central Office in Turkey
Immunocytometric Systems in Clinical and Research Flow Cytometry 2005, September	Heidelberg, Germany BD Biosciences Eastern Europe Central Office
Educational Training in Clinical Immunology and Haematology 2002, January 12 – 2002, March 5	London, UK University College London
Educational Training in Immunological Methods 1998, 5 October - 1998 , 24 December	London, UK University College London
Educational Training in General Immunology 1998, 2 March - 1998 , 25 May	London, UK University College London

PARTICIPATION IN SCIENTIFIC MEETINGS AND CONFERENCES:

1. European Academy of Allergy and Immunology Congress: “Effect of CD180 ligation or CD180/IgM sequential ligation on signalling pathways in chronic lymphocytic leukaemia cells”, Geneva, Switzerland, 2012
2. XIV International Workshop on Chronic Lymphocytic Leukemia:” Pro-Survival Signalling in ‘Responder' CLL Cells After Ligation of CD180”, Houston, USA, October, 2011Top of Form
3. 52nd American Society of Haematology (ASH) Annual Meeting and Exhibition:“B-Cell Chronic Lymphocytic Leukemia (B-CLL) Cells Unresponsive to CD180 Ligation Fail to Respond to Anti-IgM Stimulation as Well”, Orlando, USA, 2010
4. XIII International Workshop on Chronic Lymphocytic Leukemia:“Ligation Of CD180 Is A Strong Activation, Proliferation And Survival Signal For B Cells From Normal Subjects And Certain B-CLL Patients”, Barcelona, Spain, 2009
5. International Workshop on Chronic Lymphocytic Leukemia (IWCLL) Meeting:“Impaired activation of B-CLL cells through CD180-ligation is associated with a significantly decreased responsiveness via CD40, IgM and IL-4”, London, UK, 2007
6. EuroSciCon meeting:“The role of TLRs in adaptive immune responses.”,London, UK, 2007
7. American Society of Haematology (ASH) Annual Meeting: “Differential expression of CD180 and sIgM by B chronic lymphocytic leukaemia cells (B-CLL) using mutated and unmutated Vh genes”,San Diego, USA, 2004
8. American Society of Haematology (ASH) Annual Meeting:“Heterogeneity of expression of Toll-like receptor RP105 (CD180) by B cell chronic lymphocytic leukaemic (B-CLL) cells”, Philadelphia, USA, 2002
9. British Society of Haematology (BSI) Meeting:”Spontaneous apoptosis of tumour cells in patients with B chronic lymphocytic leukaemia (B-CLL) is inhibited by CD40 ligation”, Harrogate, UK, 2000

AWARDS:

2012 – Abstract Prize for an outstanding abstract presentation at EAACI Congress 2012, 16-20 June, Geneva, Switzerland

2008, January – 2008 December – Beritashvili Stipend for Young Scientists, Georgian National Science Foundation (GNSF)

2004, December -2007 December – Stipend of the President of Georgia for Young Scientists, Georgian National Science Foundation (GNSF)

1995 – 2000 – OSGF (Soros’ Foundation) Stipend for Outstanding Students

PUBLICATIONS:

Number of publications:  29

1.     Kulikova N., Lydyard P.M., Vispute K., Steele A., Tsertsvadze T., Botschorishvili E.,  Mitskevich N., Nathwani N., Clark E.A., Porakishvili^. N. Effect of CD180 ligation or CD180/IgM sequential ligation on signalling pathways in “Responder” CLL cells. EAACI Congress, Geneva, Swirzerland, 2012 (abstract)

2. Porakishvili N., Lydyard P.M., Vispute K., Memon A., Steele A., Kulikova N., Nathwani A., Rai K.R., Damle R.N., Clark E.A., Chiorazzi N. Pro-survival signalling in “Responder” CLL cells following ligation of CD180. Clin Lymph Myel Leuk, 2011,  11 (Suppl 2): S164-S165  (abstract)
3. Kulikova N., Botchorishvili E., Tsertsvadze T., Tsagareishvili P., Sereda L., Tevzadze M., Ghirdaladze M., Gachechiladze N., Mitskevichi N., Lydyard P., Porakishvili N. CD180 surface expression by B-chronic lymphocytic leukemia (B-CLL) cells correlates with the percentages of smudge cells in B-CLL blood smears. The 4th International IMBG Conference for young scientists, 2011 (abstract)
4. Porakishvili N., Memon A., Vispute K., Kulikova N., Clark E., Rai K., Natwani A., Damle R., Chiorazzi N. and Lydyard P. CD180 functions in activation, survival and cycling of B chronic lymphocytic leukaemia cells. Br J Haematol. , 2011, 153(4):486-98. 
5. Asatiani N., Abuladze M., Kartvelishvili T., Kulikova N., Asanishvili L., Holman H.-Y., Sapojnikova N. Response of antioxidant defense system to chromium (VI)-induced cytotoxicity in human diploid cells. Biometals, 2010, 23:161-172
6. Porakishvili N., Lydyard P., Bremser A., Vispute K., Memon A., Kulikova N., Clarke E., Rai K.R., Nathwani A.,  Damle R.N., Chiorazzi N. B-cell lymphocytic leukemia (B-CLL) cells unresponsive to CD180 ligation fail to respond to anti-IgM stimulation as well. Leukemia&Lymphoma, 2010, 3582 (abstract)
7.  Porakishvili N., Memon A., Atik C., Clark E.A., Kulikova N., Nathwani A., Kaufman M.S., Rai K.R., Damle R.N., Chiorazzi N., Lydyard P.M. Ligation of CD180 is a strong activation, proliferation and survival signal for B cells from normal subjects and certain B-CLL patients. Haematologica,2009, 94(s3), s37 (abst.4.31) ( XIII iwCLL 2009, Barcelona, Spain (abstract))
8. Kulikova N., Tsertsvadze T., Tsagareishvili P.,Sereda L., Tevzadze M., Kardava L., Gachechiladze N., Lukhumaidze M., Ghirdaladze M., Amaglobeli N., Porakishvili N. Association between seropositivity for cytomegalovirus (CMV) and CD4+ Cytotoxic T cells expansions in patients with B-cell chronic lymphocyte leukaemia (B-CLL) and healthy controls. Proceedings of the Georgian Academy of Sciences, 2009, Vol. 7, No. 3-4, 41-46
9. Abuladze M, Asatiani N, Kartvelishvili T, Kuikova N, Sapojnikova N. The comparison of chromium mediated cell responses in two different human cell lines. Metal Ions in Biology and Medicine,2008, p.281-287
10.  Porakishvili N., Memon A., Kulikova N., Manoussaka M., Lydyard P.M.The role of TLRs in adaptive immune responses. EuroSciCon (Toll-like receptors - Investigating innate immunity & infection)  25th Sept 2007 (http://www.euroscicon.com/archivehighlights.html) (abstract)
11. Memon A, Lydyard PM, Kulikova N,  Nathwani A, Clark EA and Porakishvili N. Impaired activation of B-CLL cells through CD180-ligation is associated with a significantly decreased responsiveness via CD40, IgM and IL-4.  XII iwCLL 2007, London, UK (abstract)
12. Kulikova N, Okujava R, Kardava L, Kartvelishvili M, Iosava G, Ghirdaladze D, Porakishvili N. Apoptosis induction in B-chronic lymphocytic leukaemia cells in vitro via ligation of CD19. Proc Geo Acad Sci. 2006, 32, 2:331-339
13. Kulikova N, Okujava R, Janikashvili N, Gabunia Kh, Mitskevichi N, Tsertsvadze T, Tsagareishvili P, Ghirdaladze D, Porakishvili N. Toll-like receptors expression profile in patients with B cell Chronic Lymphocytic Leukaemia (B-CLL). Proc Geo Scad Sci, 2006, 32, 3:523-530
14. Jordania Ts. , Kulikova N. , Okujava R. , Shelegia K. , Sereda L. , Tevzadze M. , Ghirdaladze M. ,  Porakishvili N. Detection of cell cycle phase distribution in B-chronic lymphocytic leukaemia (B-CLL) cells after in vitro ligation with anti-CD5 or anti-CD19 monoclonal antibodies. Proc Geo Scad Sci, 2006, 32, 3: 597-603
15. Porakishvili N, Kulikova N, Jewell AP, Yoinou PY, Nedelec S, Yong K, Nathwani A, Heelan B, Duke V, Hamblin TJ, Wallace P, Ely P, Clark EA and Lydyard PM. Differentiational expression of CD180 by B chronic lymphocytic leukaemic cells with mutated and unmutated Ig Vh genes. Br J Haematol. , 2005, 131, p. 313-319
16. Porakishvili N., Kulikova N., Manoussaka M, Jewell A.P.,Youinou P.Y., Nedelec S., Yong K., Nathwani A., Heelan B., Duke V., Hamblin T.J., Wallace P., Ely P., Clark E.A., Lydyard P.M. Differential expression of CD180 and sIgM by B chronic lymphocytic leukaemia cells (B-CLL) using mutated and unmutated Vh genes.  Blood, 2004, Vol.104, No.11, 768a (abstract)
17. Porakishvili N., Manoussaka M., Kulikova N., Walton A.J., Nathwani A., Stevenson F., Clark E.A., Lydyard P.M. Activation and proliferation of B cell chronic lymphocytic leukaemic (B-CLL) cells through anti-CD180, anti-CD40 and IL-4. Blood, 2004,Vol.104, No.11, 277b (abstract)
18. Asatiani N., Sapojnikova N., Abuladze M., Kartvelishvili T., Kulikova N., Kiziria E., Namchevadze E., Holman H.-Y. Effects of Cr(VI) long-term and low-dose action on mammalian antioxidant enzymes (an in vitro study). Journal of Inorganic Biochemistry, 2004, 98, 490-496
19. Akhobadze T., Jordania Ts., Kulikova N., Kardava L., Gachechiladze N. , Ghirdaladze D., Iosava G., Porakishvili N. Patterns of the expression of CD32 and CD64 on monocytes of patients with B cell chronic lymphocytic leukemia. Proceedings of the Georgian Academy of Sciences, Biol. Ser. B, 2004 Vol.2, No.3-4 , 39-41
20. Porakishvili N, Kulikova N , Jewell AP, Younou PY, Yong K, Nathwani A, Heelan B, Duke V, Stevenson FK, Hamblin TJ, Wallace P, Ely P, Clark EA, Lydyard PM. Preferential expression of CD180 by B cell chronic lymphocytic leukemia (B-CLL) cells using IgVh mutated than those using IgVh unmutated genes. Leukemia&Lymphoma, 2003, 153(abstract)
21. Akhobadze T., Kapanadze T., Baloyan D., Kulikova N., Kardava L., Kartvelishvili M., Jordania Ts., Gabunia Kh., Porakishvili N. Abberant expression of Fc γ-receptor (CD64) by monocytes and neutrophils from patients with B cell chronic lymphocytic leukemia. Proceedings of the Georgian Academy of Sciences, Biol. Ser. B, 2003 Vol.1, No.1-2 , 39-41
22. Porakishvili N., Kulikova N., Sundar S., Jewell A.P., Youinou P.Y. , Nedelec S., Yong K., Stevenson F., Hamblin T.J., Wallace P., Ely P., Clark E.A., Lydyard P.M. Heterogeneity of expression of Toll-like receptor RP105 (CD180) by B cell chronic lymphocyticleukaemic (B-CLL) cells. Blood, 2002, suppl 52. (abstract)
23. Gabunia Kh., Kardava L., Kulikova N., Jordania Ts., Burjanadze L., Sereda L., Porakishvili N. Phagocytosis and the regulation of apoptosis in B-chronic lymphocytic leukaemia. Bulletin of the Georgian Academy of Sciences, 2002, 166: 1.
24. Porakishvili N , Mageed R, Jamin C, Pers J-O, Kulikova N, Renaudineau Y, Lydyard PM, Youinou PY. Recent progress in the understanding of B cell functions in autoimmunity. Scand J Immunol., 2001, 54: 30-38.
25. Kardava L., Kulikova N., Tevzadze M., Gabunia Kh ., Barbakadze V., Ghirdaladze D., Iosava G., Porakishvili N. The cell cycle progression of B-chronic lymphocytic leukaemia cells in vitro. Proceedings of the Georgian Academy of Sciences, 2001, 27:4-6.
26. Kulikova N. , Lydyard P.M., Porakishvili N. Induction of apoptosis by anti-CD5 monoclonal antibodies in B-CLL cells correlates with the level of CD79b expression. Proceedings of the Georgian Academy of Sciences, 2001, 27: 363-368.
27. Porakishvili N., Kulikova N., Kalber T., Jewell A., Patterson K., Yong K., Youinou P.Y., .Lydyard P.M. Spontaneous apoptosis of tumour cells in patients with B chronic lymphocytic leukaemia (B-CLL) is inhibited by CD40 ligation. Immunology, 2000, 101, suppl 1, 26. abstract)
28. Porakishvili N, Kulikova N , Elliott S, Jewell AP, Patterson K, Yong K, Pers J-O, Youinou P, Lydyard PM. Apoptosis of leukaemic cells in patients with B chronic Lymphocytic Leukaemia (B-CLL) induced by the ligation of CD5. Blood, 1999, 94: 10, Suppl. 1, 313b. abstract)
29. Porakishvili N, Kulikova N. Correlation of the expression of some cell surface molecules on tumour cells of patients with B Chronic lymphocytic leukaemia (B-CLL). Int J Immunorehab, 1999, 14, 73.(abstract)

LANGUAGES:

1.  Georgian and Russian (Mother tongues);

 2. English (fluent);

MEMBERSHIP:

European Association of Allergology and Clinical Immunology  (EAACI)– Junior Member

International Clinical Cytometry Society (ICCS) – Member

International Society of Advanced Cytometry (ISAC) – Member

Georgian Society of Cytometry (GSC) – Founder, Head

MARITAL STATUS: married with two daughters, born on August,2002 and November,2008